Sport mouthguards are dental devices used by athletes who need oral protection during sports activities. Ethylene Vinyl Acetate (EVA), a polymeric polymer of ethylene and vinyl acetate, has become widely accepted as a mouthguard material. There are several issues related to the use of an oral protective device, especially for professional athletes who are subjected to several hours of workout everyday [[ 1]–[ 5]]. Glass et al. [[ 6]] reported an increase of oral lesions in athletes who had used stock mouthguards for an entire agonistic season. Furthermore, mouthguard conditions after wear may facilitate disease transmission (e.g. endocarditis). In fact, the continuous solutions caused by worn mouthguards would facilitate the entry into the bloodstream of all the microorganisms present on mouthguard surfaces. This condition occurs when jagged and sharp areas of the posterior regions of the mouthguards are in close proximity to the pterygoid venous plexus. revention of oral damage, caused by pathogen's flora to hard and soft tissues, can be done by following the basic oral hygiene rules before sport activity and cleaning the device after each use [[10]]. Preservation and cleaning of the mouthguard are often overlooked by athletes who easily tend to abandon this task. Namba et al. [[11]] conducted an interview to 22 rugby players, with an appropriate level of school education, to understand their habits and attitudes towards the hygiene and the use of mouthguards. Only two of them reported to have been correctly guided by a sport dentist on methods to sanitize their mouthguard. When asked if they performed some type of mouthguard cleaning, only one answered that always cleaned it with water.